Product nameAlexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control
ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm
Specificityab178002 was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. ab178002 is a suitable control for Human, Rat, Mouse, Monkey, others untested.
Tested applicationsSuitable for: ICC/IFmore details
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Light chain typekappa
Our Abpromise guarantee covers the use of ab178002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
Immunocytochemistry data for ab178002. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178002 at 1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 1 μg/mL (C).
Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C. Cells were blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3X with 1%GS and DAPI was added for 10 minutes in 1%GS.
ab178002 has not yet been referenced specifically in any publications.