Key features and details
- Rabbit polyclonal to ALG12
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ALG12 antibody
DescriptionRabbit polyclonal to ALG12
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human ALG12 (internal sequence). Gene Accssion: BC001729
Database link: Q9BV10
- PC3 cell lysate and Human thyroid cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/100.|
|WB||1/200 - 1/1000. Predicted molecular weight: 55 kDa.|
FunctionAdds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation.
Tissue specificityExpressed in fibroblasts.
PathwayProtein modification; protein glycosylation.
Involvement in diseaseDefects in ALG12 are the cause of congenital disorder of glycosylation type 1G (CDG1G) [MIM:607143]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
Sequence similaritiesBelongs to the glycosyltransferase 22 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 6-mannosyltransferase antibody
- ALG12 antibody
- ALG12_HUMAN antibody
Anti-ALG12 antibody (ab197830) at 1/200 dilution + PC3 cell lysate at 40 µg
Anti-rabbit IgG at 1/8000 dilution
Predicted band size: 55 kDa
Exposure time: 10 seconds
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ALG12 with ab197830 at 1/20 dilution.
ab197830 has not yet been referenced specifically in any publications.