Overview

  • Product name

    Anti-ALG12 antibody
  • Description

    Rabbit polyclonal to ALG12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human ALG12 (internal sequence). Gene Accssion: BC001729
    Database link: Q9BV10

  • Positive control

    • PC3 cell lysate and Human thyroid cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/100.
WB 1/200 - 1/1000. Predicted molecular weight: 55 kDa.

Target

  • Function

    Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation.
  • Tissue specificity

    Expressed in fibroblasts.
  • Pathway

    Protein modification; protein glycosylation.
  • Involvement in disease

    Defects in ALG12 are the cause of congenital disorder of glycosylation type 1G (CDG1G) [MIM:607143]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • Sequence similarities

    Belongs to the glycosyltransferase 22 family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6-mannosyltransferase antibody
    • ALG12 antibody
    • ALG12_HUMAN antibody
    • Asparagine linked glycosylation 12 alpha 1 6 mannosyltransferase homolog antibody
    • Asparagine linked glycosylation protein 12 homolog antibody
    • Asparagine-linked glycosylation protein 12 homolog antibody
    • CDG1G antibody
    • Dol P Man:Man(7)GlcNAc(2) PP Dol alpha 1 6 mannosyltransferase antibody
    • Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1 antibody
    • Dolichyl P Man:Man(7)GlcNAc(2) PP dolichyl alpha 1 6 mannosyltransferase antibody
    • Dolichyl P mannose:Man 7 GlcNAc 2 PP dolichyl alpha 6 mannosyltransferase antibody
    • Dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1 antibody
    • ECM39 antibody
    • hALG12 antibody
    • Mannosyltransferase ALG12 homolog antibody
    • Membrane protein SB87 antibody
    • MGC111358 antibody
    • MGC3136 antibody
    • PP14673 antibody
    see all

Images

  • Anti-ALG12 antibody (ab197830) at 1/200 dilution + PC3 cell lysate at 40 µg

    Secondary
    Anti-rabbit IgG at 1/8000 dilution

    Predicted band size: 55 kDa


    Exposure time: 10 seconds


    Gel: 8%SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ALG12 with ab197830 at 1/20 dilution.

References

ab197830 has not yet been referenced specifically in any publications.

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