Product nameAnti-ALIX antibody [3A9]
See all ALIX primary antibodies
DescriptionMouse monoclonal [3A9] to ALIX
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein (GST-tag) corresponding to Human ALIX (N terminal).
Database link: Q8WUM4
- WB: HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab117600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Do not perform antigen retrieval.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionClass E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
Sequence similaritiesContains 1 BRO1 domain.
Cellular localizationCytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
- Information by UniProt
- AIP1 antibody
- ALG 2 interacting protein 1 antibody
- ALG-2-interacting protein 1 antibody
This product has been referenced in:
- Tanaka Y et al. Adiponectin promotes muscle regeneration through binding to T-cadherin. Sci Rep 9:16 (2019). Read more (PubMed: 30626897) »
- Zou W et al. Exosome Release Is Regulated by mTORC1. Adv Sci (Weinh) 6:1801313 (2019). Read more (PubMed: 30775228) »