Recombinant Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)
![Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-349651-anti-alix-antibody-epr15314-33-n-terminal-western-blot-knockout-hek293-pdcd6ip-hela-human-lysates.jpg "Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15314-33] to ALIX - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ALIX antibody [EPR15314-33] - N-terminal
See all ALIX primary antibodies -
Description
Rabbit monoclonal [EPR15314-33] to ALIX - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, K562 and 293 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15314-33 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. -
Sequence similarities
Contains 1 BRO1 domain. -
Cellular localization
Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells. - Information by UniProt
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Database links
- Entrez Gene: 10015 Human
- Omim: 608074 Human
- SwissProt: Q8WUM4 Human
- Unigene: 475896 Human
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Alternative names
- AIP1 antibody
- ALG 2 interacting protein 1 antibody
- ALG-2-interacting protein 1 antibody
see all
Images
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Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)All lanes : Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : ALIX knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab186728 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab186728 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in ALIX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ALIX knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab186728 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-220015-anti-alix-antibody-epr15314-33-n-terminal-western-blot.jpg)
Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)All lanes : Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
![Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-3-benefits-of-recombinant-antibodies.png)
Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab186728 has been referenced in 3 publications.
- Bai S et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
- Mussack V et al. Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research. Biomol Detect Quantif 17:100089 (2019). PubMed: 31194192
- Hermann S et al. Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera. J Extracell Vesicles 8:1670935 (2019). PubMed: 31632620