Product nameAlizarin red S, histochemical stain
DescriptionHistochemical stain for calcium.
- 3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
- Alizarin Carmine
- Alizarin Red, water soluble
Anthraquinone dye used to determine, quantitatively by colorimetry, the presence of calcific deposition by cells of an osteogenic lineage. Has also been used as a histochemical stain for calcium in cells of non-osteogenic lineage and as a colorimetric pH indicator.
Chemical name9,10-Dihydro-3,4-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid monosodium salt
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 1 mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Verma D et al. Osteoblast adhesion, proliferation and growth on polyelectrolyte complex-hydroxyapatite nanocomposites. Philos Trans A Math Phys Eng Sci 368:2083-97 (2010). Read more (PubMed: 20308116) »
- Bertoni L et al. Leptin increases growth of primary ossification centers in fetal mice. J Anat 215:577-83 (2009). Read more (PubMed: 19682137) »
- Paul H et al. Alizarin red S staining as a screening test to detect calcium compounds in synovial fluid. Arthritis Rheum 26:191-200 (1983). Read more (PubMed: 6186260) »
- Lievremont M et al. Use of alizarin red S for histochemical staining of Ca2+ in the mouse; some parameters of the chemical reaction in vitro. Acta Anat (Basel) 114:268-80 (1982). Read more (PubMed: 7158284) »
- Puchtler H et al. On the history and mechanism of alizarin and alizarin red S stains for calcium. J Histochem Cytochem 17:110-24 (1969). Read more (PubMed: 4179464) »