Product nameAnti-ALK-1 antibody [EPR22985-108]
See all ALK-1 primary antibodies
DescriptionRabbit monoclonal [EPR22985-108] to ALK-1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse ALK-1 aa 1-150. The exact sequence is proprietary.
Database link: Q61288
- WB: Recombinant Mouse ALK-1 Fc Chimera protein; Mouse lung and placenta lysates; rat lung lysates; bEnd.3 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab263902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 63, 56 kDa (predicted molecular weight: 56 kDa).|
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.
Involvement in diseaseDefects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
- Information by UniProt
- Activin A receptor antibody
- Activin A receptor type II like 1 antibody
- Activin A receptor, type II like kinase 1 antibody
All lanes : Anti-ALK-1 antibody [EPR22985-108] (ab263902) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat placenta lysate
Lane 5 : bEnd.3 (mouse brain endothelioma) whole cell lysate
Lane 6 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56,63 kDa why is the actual band size different from the predicted?
Blockinmg and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
There are two isoforms. Different isoforms show different content in different tissues.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11927558, 7488127).
Negative control: MEF (PMID: 11927558).
Anti-ALK-1 antibody [EPR22985-108] (ab263902) at 1/1000 dilution + Recombinant Mouse ALK-1 Fc Chimera protein (aa23-119), 20 ng
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263902 has not yet been referenced specifically in any publications.