Overview

  • Product name

    Anti-ALK-1 antibody [EPR4074]
    See all ALK-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4074] to ALK-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ALK-1 aa 450 to the C-terminus.
    Database link: P37023
    (Peptide available as ab190772)

  • Positive control

    • JAR, Daudi or Jurkat whole cell lysate (ab7899)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.
    • Involvement in disease

      Defects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activin A receptor antibody
      • Activin A receptor type II like 1 antibody
      • Activin A receptor, type II like kinase 1 antibody
      • Activin receptor like kinase 1 antibody
      • Activin receptor-like kinase 1 antibody
      • ACVL1_HUMAN antibody
      • ACVRL1 antibody
      • ACVRLK1 antibody
      • ALK-1 antibody
      • ALK1 antibody
      • HHT antibody
      • HHT2 antibody
      • ORW2 antibody
      • Osler Rendu Weber syndrome 2 antibody
      • Serine/threonine protein kinase receptor R3 antibody
      • Serine/threonine-protein kinase receptor R3 antibody
      • SKR3 antibody
      • TGF B superfamily receptor type I antibody
      • TGF-B superfamily receptor type I antibody
      • TSR-I antibody
      • TSR1 antibody
      see all

    Images

    • All lanes : Anti-ALK-1 antibody [EPR4074] (ab108207) at 1/1000 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : mouse kidney lysate
      Lane 3 : rat brain lysate
      Lane 4 : rat kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-ALK-1 antibody [EPR4074] (ab108207) at 1/1000 dilution (purified)

      Lane 1 : JAR cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-ALK-1 antibody [EPR4074] (ab108207) at 1/1000 dilution (unpurified)

      Lane 1 : JAR cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    References

    This product has been referenced in:

    • Li Q  et al. Downregulation of miR-98 contributes to hypoxic pulmonary hypertension by targeting ALK1. Mol Med Rep 20:2167-2176 (2019). Read more (PubMed: 31322216) »
    • Zhu W  et al. Induction of Brain Arteriovenous Malformation Through CRISPR/Cas9-Mediated Somatic Alk1 Gene Mutations in Adult Mice. Transl Stroke Res N/A:N/A (2018). Read more (PubMed: 30511203) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Rat liver tissue)
    Gel Running Conditions
    Reduced Denaturing (9% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Rat liver tissue
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 25 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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