Overview

  • Product name
    Anti-ALK-1 antibody [MM0015-8G33]
    See all ALK-1 primary antibodies
  • Description
    Mouse monoclonal [MM0015-8G33] to ALK-1
  • Host species
    Mouse
  • Specificity
    This antibody recognizes human ALK-1.
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human ALK-1 (extracellular).
    Database link: P37023

Applications

Our Abpromise guarantee covers the use of ab51870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/100 - 1/800. Predicted molecular weight: 63 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. May bind activin as well.
  • Involvement in disease
    Defects in ACVRL1 are the cause of hereditary hemorrhagic telangiectasia type 2 (HHT2) [MIM:600376]; also known as Osler-Rendu-Weber syndrome 2 (ORW2). HHT2 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary, cerebral and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin A receptor antibody
    • Activin A receptor type II like 1 antibody
    • Activin A receptor, type II like kinase 1 antibody
    • Activin receptor like kinase 1 antibody
    • Activin receptor-like kinase 1 antibody
    • ACVL1_HUMAN antibody
    • ACVRL1 antibody
    • ACVRLK1 antibody
    • ALK-1 antibody
    • ALK1 antibody
    • HHT antibody
    • HHT2 antibody
    • ORW2 antibody
    • Osler Rendu Weber syndrome 2 antibody
    • Serine/threonine protein kinase receptor R3 antibody
    • Serine/threonine-protein kinase receptor R3 antibody
    • SKR3 antibody
    • TGF B superfamily receptor type I antibody
    • TGF-B superfamily receptor type I antibody
    • TSR-I antibody
    • TSR1 antibody
    see all

Images

  • ab51870 at 1/50 dilution staining ALK-1 in Human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Overlay histogram showing JEG3 cells stained with ab51870 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51870, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab991361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Tengroth L  et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). Read more (PubMed: 29367682) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Placenta
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jun 27 2013

Answer

Thank you very much for getting back to me and for confirming the construct details.

If you have a chance, it would be still worth running a good positive control (cytospin preparation from A549 cells or tissue section from placenta) which endogenously express TGF beta Receptor I. This product was launched in March 2006 so it has been on the market for several years.

There are 8 specific publications and the following papers have been used in immunostaining:

Devaux Y et al. Transforming growth factor ß receptor 1 is a new candidate prognostic biomarker after acute myocardial infarction. BMC Med Genomics 4:83 (2011). IHC-Fr ; Rat .





Sounni NE et al. Stromal regulation of vessel stability by MMP14 and TGFbeta. Dis Model Mech 3:317-32 (2010). IHC-Fr ; Mouse.





Beckham JD et al. Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection. J Virol 83:5035-45 (2009). IHC-P ; Mouse .



I could offer you a new vial of ab31013 free of charge but we are waiting for a new stock. We have two alternative products against the same target TGF beta Receptor I. If you wish test either of them, I can send you a new vial free of charge.

https://www.abcam.com/ab155258: Anti-TGF beta Receptor I antibody

https://www.abcam.com/ab51870: Anti-TGF beta Receptor I antibody [MM0015-8G33]

Let me know how you wish to proceed with your enquiry. I look forward to hearing from you soon.

Read More

Answer

Thank you for your enquiry which has been forwarded to our Scientific Support team.

I would like to reassure you that our antibodies have been tested successfully and would be covered by our 6 month guarantee in the applications listed on the individual datasheets. Therefore, any TGF beta Receptor Iantibody tested in the species and application you are using should be suitable for your experiments.

I can confirm that ab49575 clone [8A11] and ab51870 clone MM0015-8G33 have both been tested in IHC on paraffin embedded sections (IHC-P).

Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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