Overview

  • Product name
    Anti-ALK antibody [5A4]
    See all ALK primary antibodies
  • Description
    Mouse monoclonal [5A4] to ALK
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).

  • Positive control
    • IHC-P: Human anaplastic lymphoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab17127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/70. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
  • Tissue specificity
    Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
  • Involvement in disease
    A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
    A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
    A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
    Neuroblastoma 3
    The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
    A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 LDL-receptor class A domain.
    Contains 2 MAM domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
    N-glycosylated.
  • Cellular localization
    Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alk antibody
    • ALK tyrosine kinase receptor antibody
    • ALK/EML4 fusion gene, included antibody
    • ALK/NPM1 fusion gene, included antibody
    • ALK_HUMAN antibody
    • anaplastic lymphoma kinase (Ki-1) antibody
    • Anaplastic lymphoma kinase antibody
    • Anaplastic lymphoma kinase Ki1 antibody
    • anaplastic lymphoma receptor tyrosine kinase antibody
    • CD 246 antibody
    • CD246 antibody
    • CD246 antigen antibody
    • EC 2.7.10.1 antibody
    • Ki 1 antibody
    • Ki1 antibody
    • mutant anaplastic lymphoma kinase antibody
    • NBLST 3 antibody
    • NBLST3 antibody
    • Tcrz antibody
    • TFG/ALK antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human anaplastic lymphoma tissue, staining ALK with ab17127.

References

This product has been referenced in:
  • Tartarone A  et al. Molecular characterization and prognostic significance of circulating tumor cells in patients with non-small cell lung cancer. J Thorac Dis 9:S1359-S1363 (2017). Read more (PubMed: 29184674) »
  • Manicone M  et al. Liquid biopsy for monitoring anaplastic lymphoma kinase inhibitors in non-small cell lung cancer: two cases compared. J Thorac Dis 9:S1391-S1396 (2017). Read more (PubMed: 29184678) »
See all 14 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer



L'anticorps anti-ALK ab17127 a été testé en IHC-Paraffine et devrait fonctionner sans problème avec un automate Benchmark XT Ventana.
Veuillez noter que nous recommandons de procéder à un démasquage de l'antigène à la chaleur et d'utiliser l'anticorps à une dilution entre 1/25 et 1/70.

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Answer

Thank you for your enquiry and interest in our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. As ab17127 has already been tested in IHC-P, I would like to reassure you that this guarantee would apply in this case. I am sorry we are therefore not able to provide any further offers in this case. Regarding ab100800Anti-HJURP antibody, as this has not yet been tested in IHC-P, you are eligible for our testing discount program.I can offer a discount off a future purchase if you buy ab100800 now, test it inIHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab100800 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab100800 so please wait for my reply before ordering. 2. Purchase ab100800 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab100800 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Please let me know if you have any questions about this offer and I would be happy to help you further.

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Answer

Merci pour ces précisions qui nous permettent de prendre les dispositions nécessaires quant au lot de ab17127 défectueux. J'ai mis en place l'envoi d'une unité de ab17127 lot GR45198-2 en remplacement du lot GR28265-2. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

Merci de nous avoir contactés. Comme expliqué par téléphone, nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (si le problème nous est communiqué dans les 6 mois qui suivent la commande). Nous pouvons donc toujours résoudre le problème que vous avez rencontré en vous remboursant, vous faire un avoir, ou vous envoyer un anticorps de remplacement. En échange, nous serions ravis si vous pouviez nous communiquer quelques détails concernant votre protocole en remplissant le questionnaire ci-joint. Ces informations sont très importantes pour nous car elles nous permettent d'investiguer la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits.

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Question
Answer

Thank you for your enquiry. The immunogen sequence is spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa). Please refer to the following publication for more information on the sequence. Detection of Anaplastic Lymphoma Kinase (ALK) and Nucleolar Protein Nucleophosmin (NPM)-ALK Proteins in Normal and Neoplastic Cells With the Monoclonal Antibody ALK1. Blood 1997 89: 1394-1404 http://bloodjournal.hematologylibrary.org/content/89/4/1394.full.pdf+html If there is anything else that I can help you with please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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