Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1] (ab49746)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG2a
Overview
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Product name
Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]
See all 6X His tag® primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag® -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WB, Dot blot, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant poly(Histidine)-tagged fusion protein.
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Positive control
- Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.097% Sodium azide
Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA -
Concentration information loading... -
Primary antibody notes
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors. -
Clonality
Monoclonal -
Clone number
HIS-1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab49746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 7 kDa. | |
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
References (3)
ab49746 has been referenced in 3 publications.
- Bottermann M et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe 25:617-629.e7 (2019). PubMed: 30926239
- Henche AL et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). PubMed: 25102813
- Hussack G et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . PubMed: 21216961
