Anti-Alkaline Phosphatase antibody (ab354)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase
- Suitable for: ELISA, IP, WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-Alkaline Phosphatase antibody
See all Alkaline Phosphatase primary antibodies -
Description
Rabbit polyclonal to Alkaline Phosphatase -
Host species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. -
Tested applications
Suitable for: ELISA, IP, WBmore details -
Species reactivity
Reacts with: Escherichia coli
Does not react with: Mammals -
Immunogen
Full length protein corresponding to Alkaline Phosphatase. Full length protein (E. coli).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Whole antiserum -
Purification notes
This product was prepared from monospecific antiserum by a delipidation and defibrination. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Alkaline phosphatase, germ cell antibody
- Alkaline phosphatase, testicular and thymus antibody
- Akp2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab354 has been referenced in 8 publications.
- Hodgkinson T et al. The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells. Sci Adv 7:N/A (2021). PubMed: 33637520
- Hemnes AR et al. BMPR2 dysfunction impairs insulin signaling and glucose homeostasis in cardiomyocytes. Am J Physiol Lung Cell Mol Physiol 318:L429-L441 (2020). PubMed: 31850803
- Asgharian-Rezaee M et al. Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells. Rep Biochem Mol Biol 9:331-337 (2020). PubMed: 33649727
- Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). PubMed: 30043216
- Tsimbouri PM et al. Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nat Biomed Eng 1:758-770 (2017). PubMed: 31015671
- Bross P et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . PubMed: 18400758
- Imperi F et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). PubMed: 18757814
- Downer R et al. The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. J Biol Chem 277:243-50 (2002). PubMed: 11684686