Overview

  • Product name
    Anti-Alkaline Phosphatase antibody
    See all Alkaline Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Alkaline Phosphatase
  • Host species
    Rabbit
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
    Does not react with: Mammals
  • Immunogen

    Full length protein (E. coli).

Properties

Applications

Our Abpromise guarantee covers the use of ab354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000 - 1/40000.
IP Use at an assay dependent dilution.
ELISA 1/8000 - 1/40000.

Target

  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Alternative names
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Images

  • ELISA - Anti-Alkaline Phosphatase antibody (ab354)
    ELISA - Anti-Alkaline Phosphatase antibody (ab354)This image is courtesy of an anonymous Abreview
    Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.

    See Abreview

  • Western blot - Anti-Alkaline Phosphatase antibody (ab354)
    Western blot - Anti-Alkaline Phosphatase antibody (ab354)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution

    All lanes : Escherichia coli Recombinant protein (Mach1)

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution

    Developed using the ECL technique.

    Observed band size: 90 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 seconds

References

This product has been referenced in:
  • Bross P  et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . Read more (PubMed: 18400758) »
  • Imperi F  et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). Read more (PubMed: 18757814) »

See all 3 Publications for this product

Publishing research using ab354? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
Votes: Highest First

ELISA abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Recombinant protein (Mach1)
Specification
Mach1
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Indirect
Read More
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Abcam user community

Verified customer

Submitted Feb 26 2010

Western blot abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (Mach1)
Loading amount
0.5 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
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Username

Abcam user community

Verified customer

Submitted Feb 19 2010

Please help with the following questions: What is the concentration of the antibody to bacterial alkaline phosphatase? Is this an affinity purified polyclonal? Are there ANY other proteins in the preparation?

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG con...

Read More

I saw an enquiry dated 7-Feb-2001 asking whether this antibody can detect secreted alk phos (SEAP). At the time, the answer was that you have no reports -- have you heard anything further about this application since?

This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species.

I'm having trouble using this product for ELISA at 2µg/ml. Please let me know the recommended conditions.

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

We want to use alkaline phosphatase antibody to determine ALP releasing in periodontal cells (Osteoblasts, PDL fibroblasts and cementoblasts). Does Alp1-ab354 test ALP expression in bone-like cells by immunohistochemistry?

No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tes...

Read More

I would like to know whether your alkaline phosphatase antibody is a marker of Plasma membrane or not.

No, this antibody is not a marker of plasma membrane.

Do you know if your Alp1 Ab can detect secreted alkaline phosphatase (SEAP)?

We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

We want to use alkaline phosphatse as a marker in E. coli cell fraction experiments. To monitor alk. phosphatase we want to use your antibody. Do you know if alk. phosphatase is expressed under all condition in E. coli (at different levels). Many than...

Read More

We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

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