Question (46855) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

Go to datasheet (ab83369)

Question

What is the best method for cell lysis when using ab83369?

Answer

The buffer contained in the kit does not contain detergents, so mechanical agitation is required to complete the process of lysing the cells by passing through a syringe tip or other utensil such as a homogenizer. The scrapper is not recommended in this case.

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