Question (55904) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

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Question

Will be extracting cells off of a scaffold prior to the assay. Do you know if a Tris buffer with Triton-X is used, will this lysate be compatible with the assay?

Answer

The Tris buffer should be fine, but the Triton-X will not be good to use. Ideally you would extract the cells off of the scaffold without lysing them, then resuspend them in the assay buffer and proceed with the protocol.

I would recommend trypsinizing the cells off the scaffold, then splitting the cells if some are needed for other assays, and resuspending a portion of the cells in the assay buffer.

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