Question (74927) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

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Question

In the protocol, the samples are prepared first and then incubated with pNPP for 60 minutes. Then the standards are prepared and incubated with ALP enzyme for 60 minutes. Does that mean the samples are incubating for another 60 minutes?

Answer

The experimental samples are incubated with pNPP for only 60 minutes before adding stop solution. That is also the case for the incubation of the pNPP standards with the ALP enzyme: 60 minutes of incubation, then stop solution.

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