Question (74967) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

Go to datasheet (ab83369)

Question

I am using kit ab83369 but the data analysis section is a bit confusing. How is the 'A' variable determined (described as 'amount of pNP generated by samples (in umol)'?

Answer

Based on the standard curve equation, y=mx+b, you have to calculate x. This number signifies umoles of pNP for 1 OD. This number is A.

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