Question (75517) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

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Question

We are using ab83369 to determine ALP in cell samples. In the assay protocol it is recommended to use a sample background control to may be used for background subtraction. What type of sample would be appropriate for this? Should this be a cellular sample of some sort, or are you referring to a colorless sample, such as water that is used solely for color subtraction?

Answer

That note refers to cell culture medium samples, and the control would be a sample of medium that has not been exposed to cells. If your samples are cell lysates, you do not need this control. Your negative control will simply be the Assay Buffer included with the kit, which is the zero standard.

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