Question (75523) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

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Question

In the manual, it is written that the ALP enzyme should reconstituted with 1 mL of buffer and in each sample we should add 10 microliter of enzyme. That means we can analyze 100 sample (not 500) with this kit because there is only one vial of enzyme inside the kit. Could you please explain in more detail how it is possible to get 500 tests out of this kit?

Answer

10ul of enzyme is used for each well for the standards only. There is 1ml of reconstituted enzyme which is equal to 100 standard wells. Each std has 6 datapoints. With the enzyme mix the standard curve can be run 16 times.
The samples do not need any enzyme since this assay is for testing ALP enzyme activity in the sample.

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