Question (76557) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

Go to datasheet (ab83369)

Question

I would appreciate if you can let me know whether you elisa kit Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369),
can identify bone alkaline phosphatase.

Answer

We have not tested the kit with bone samples, but there is no reason for the kit to not work with bone samples. Grinding and homogenization would be critical. The following two references used ab83369 to detect alkaline phosphatase produced in bone tissue engineering applications, so it seems the kit was detecting alkaline phosphatase produced by osteogenic progenitor cells:



http://www.ncbi.nlm.nih.gov/pubmed/23804651?dopt=Abstract

http://www.ncbi.nlm.nih.gov/pubmed/23022346?dopt=Abstract



If you need bone alkaline phosophatase antibodies we carry the two antibodies below, which are both tested and guaranteed to work in ELISA in the species listed on their datasheets for 12 months after your purchase:



https://www.abcam.com/products?keywords=bone+alkaline+phosphatase

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