Question (76720) | Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)

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Question


i have a question about ALP kit (ab83369). For cells preparation before test, cells don't need to be sonicated? Cells suspension must be just washed and centrifuged at top speed at 4oC. Exactly?

Answer


To prepare the samples they should not necessarily require sonication, so I can recommend to follow the protocol.
If there are difficulties lysing the sample, then sonication can be tried. But it is not usually required.

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