Alkaline Phosphatase Assay Kit (Fluorometric - Near Infrared) (ab138887)
- Datasheet
- References
- Protocols
Overview
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Product name
Alkaline Phosphatase Assay Kit (Fluorometric - Near Infrared)
See all Alkaline phosphatase kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Cell culture extracts -
Assay type
Enzyme activity (quantitative) -
Sensitivity
= 0.3 mU/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Alkaline Phosphatase Assay kit (Fluorometric-Near Infrared) (ab138887) uses our SunRed Dye based substrate. The weakly fluorescent SunRed Dye phosphate is sensitive to phosphatase-induced hydrolysis, giving the SunRed Dye fluorophore that possesses intense red fluorescence. Upon phosphatase-induced hydrolysis, the SunRed Dye phosphate solution has its absorption blue-shifted more than 100 nm. The maximum absorption of SunRed Dye fluorophore at 633 nm makes this substrate an ideal NIR probe that can be readily detected with many fluorescence instrument systems often equipped with Cy5 settings. Based on the near infrared fluorescence of SunRed Dye fluorophore, the signal can be easily read by a fluorescence microplate reader at Ex/Em = ~630/660 nm.
ab138887 has been used for the high throughput screening of protein phosphatase inhibitors due to its low interference from biological samples. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
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Notes
Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Alkaline Phosphatase Standard 1 x 10 units Assay Buffer 1 x 25ml SunRed Dye Substrate (light sensitive) 1 vial -
Research areas
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Relevance
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor. -
Alternative names
- Alkaline phosphatase intestinal
- Alkaline phosphatase liver/bone/kidney
- Alkaline phosphatase liver/bone/kidney isozyme
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Images
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Sample Standard Curve for Alkaline phosphataseAlkaline phosphatase dose response was measured with the Alkaline Phosphatase Assay Kit (Fluorometric –Near Infrared) in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices). As low as 0.3 mU/mL alkaline phosphatase can be detected with 60 minutes incubation (n=3).
Protocols
References
ab138887 has not yet been referenced specifically in any publications.
