Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Cell culture supernatant
- Sensitivity: 0.3 mU/ml
Product nameAlkaline Phosphatase Assay Kit (Fluorometric - Near Infrared)
See all Alkaline phosphatase kits
Sample typeCell culture supernatant, Cell culture extracts
Assay typeEnzyme activity (quantitative)
Sensitivity= 0.3 mU/ml
Species reactivityReacts with: Mammals, Other species
Alkaline Phosphatase Assay kit (Fluorometric-Near Infrared) (ab138887) uses our SunRed Dye based substrate. The weakly fluorescent SunRed Dye phosphate is sensitive to phosphatase-induced hydrolysis, giving the SunRed Dye fluorophore that possesses intense red fluorescence. Upon phosphatase-induced hydrolysis, the SunRed Dye phosphate solution has its absorption blue-shifted more than 100 nm. The maximum absorption of SunRed Dye fluorophore at 633 nm makes this substrate an ideal NIR probe that can be readily detected with many fluorescence instrument systems often equipped with Cy5 settings. Based on the near infrared fluorescence of SunRed Dye fluorophore, the signal can be easily read by a fluorescence microplate reader at Ex/Em = ~630/660 nm.
ab138887 is designed to accommodate high throughput screening of protein phosphatase inhibitors due to its low interference from biological samples. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Alkaline Phosphatase Standard 1 x 10 units Assay Buffer 1 x 25ml SunRed Dye Substrate (light sensitive) 1 vial
RelevanceAlkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.
Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
- Alkaline phosphatase intestinal
- Alkaline phosphatase liver/bone/kidney
- Alkaline phosphatase liver/bone/kidney isozyme
ab138887 has not yet been referenced specifically in any publications.