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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Bone
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Alkaline Phosphatase Assay Kit (Fluorometric - Near Infrared) (ab138887)

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  • Protocol Booklet
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Sample Standard Curve for Alkaline phosphatase

    Key features and details

    • Assay type: Enzyme activity (quantitative)
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Cell culture extracts, Cell culture supernatant
    • Sensitivity: 0.3 mU/ml

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    Overview

    • Product name

      Alkaline Phosphatase Assay Kit (Fluorometric - Near Infrared)
      See all Alkaline phosphatase kits
    • Detection method

      Fluorescent
    • Sample type

      Cell culture supernatant, Cell culture extracts
    • Assay type

      Enzyme activity (quantitative)
    • Sensitivity

      = 0.3 mU/ml
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Alkaline Phosphatase Assay kit (Fluorometric-Near Infrared) (ab138887) uses our SunRed Dye based substrate. The weakly fluorescent SunRed Dye phosphate is sensitive to phosphatase-induced hydrolysis, giving the SunRed Dye fluorophore that possesses intense red fluorescence. Upon phosphatase-induced hydrolysis, the SunRed Dye phosphate solution has its absorption blue-shifted more than 100 nm. The maximum absorption of SunRed Dye fluorophore at 633 nm makes this substrate an ideal NIR probe that can be readily detected with many fluorescence instrument systems often equipped with Cy5 settings. Based on the near infrared fluorescence of SunRed Dye fluorophore, the signal can be easily read by a fluorescence microplate reader at Ex/Em = ~630/660 nm.


       


      ab138887 is designed to accommodate high throughput screening of protein phosphatase inhibitors due to its low interference from biological samples. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

    • Notes

      Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 500 tests
      Alkaline Phosphatase Standard 1 x 10 units
      Assay Buffer 1 x 25ml
      SunRed Dye Substrate (light sensitive) 1 vial
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Bone
      • Kits/ Lysates/ Other
      • Kits
      • IHC Kits
      • Developmental Biology
      • Organogenesis
      • Skeletal development
      • Bone
      • Kits/ Lysates/ Other
      • Kits
      • Cell Signaling Kits
      • Phosphatase assay kits
    • Relevance

      Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.
    • Cellular localization

      Cell membrane; Lipid-anchor, GPI-anchor.
    • Alternative names

      • Alkaline phosphatase intestinal
      • Alkaline phosphatase liver/bone/kidney
      • Alkaline phosphatase liver/bone/kidney isozyme
      • Alkaline phosphatase tissue nonspecific isozyme
      • Alkaline phosphomonoesterase
      • ALPI
      • ALPL
      • AP TNAP
      • Glycerophosphatase
      • HOPS
      • IAP
      • Intestinal alkaline phosphatase
      • Kasahara isozyme
      • Liver/bone/kidney type alkaline phosphatase
      • Tissue nonspecific ALP
      • TNAP
      • TNSALP
      see all

    Associated products

      Images

      • Sample Standard Curve for Alkaline phosphatase
        Sample Standard Curve for Alkaline phosphatase
        Alkaline phosphatase dose response was measured with the Alkaline Phosphatase Assay Kit (Fluorometric –Near Infrared) in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices). As low as 0.3 mU/mL alkaline phosphatase can be detected with 60 minutes incubation (n=3).

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab138887? Please let us know so that we can cite the reference in this datasheet.

      ab138887 has not yet been referenced specifically in any publications.

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