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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Alkaline Phosphatase Assay Kit (Luminometric) (ab233466)

  • Datasheet
  • Protocol Booklet
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Example Standard Curve

    Key features and details

    • Assay type: Quantitative
    • Detection method: Luminescent
    • Platform: Microplate reader
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Alkaline Phosphatase Assay Kit (Luminometric)
      See all Alkaline Phosphatase kits
    • Detection method

      Luminescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Quantitative
    • Product overview

      Alkaline Phosphatase Assay Kit (ab233466) uses D-luciferin phosphate as the luminogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions and cells.


      D-luciferin phosphate is not recognized by luciferase until its phosphate group is removed to give luciferin. This kit provides all the essential components with an optimized assay protocol which is compatible with HTS liquid-handling instruments. The assay can be readily performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.


      The high sensitivity makes the kit ideal for the assays that require low detection limit.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      Alkaline Phosphatase Standard 1 vial
      Assay Buffer 1 x 5ml
      Phosphatase Substrate 1 vial
      Reaction Buffer 1 x 5ml
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Phosphatases
      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • Alkaline Phosphatase
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • Phosphatases
    • Sequence similarities

      Belongs to the alkaline phosphatase family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P09923 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Alkaline phosphatase, germ cell
      • Alkaline phosphatase, testicular and thymus
      • Akp2
      • Alkaline phosphatase intestinal
      • Alkaline phosphatase intestinal precursor
      • Alkaline phosphatase liver/bone/kidney
      • Alkaline phosphatase placental
      • Alkaline phosphatase placental like
      • Alkaline phosphatase placental like 2
      • Alkaline phosphatase placental type
      • Alkaline phosphatase, liver
      • Alkaline phosphatase, tissue-nonspecific
      • Alkaline phosphomonoesterase
      • ALP
      • ALP 1
      • ALP I
      • ALP L
      • ALP P
      • ALPG
      • Alpi
      • ALPL
      • ALPP
      • ALPPL
      • ALPPL2
      • AP TNAP
      • FLJ40094
      • GCAP
      • Germ cell alkaline phosphatase
      • Glycerophosphatase
      • HOPS
      • IAP
      • Intestinal alkaline phosphatase
      • Intestinal-type alkaline phosphatase
      • Kasahara isozyme
      • Nagao isozyme
      • OTTHUMP00000164357
      • Placental alkaline phosphatase
      • Placental like alkaline phosphatase
      • PLAP
      • PLAP 1
      • PLAP like
      • PPBI_HUMAN
      • Regan isozyme
      • Testicular and thymus alkaline phosphatase
      • Tissue non specific alkaline phosphatase
      • Tissue nonspecific ALP
      • TNAP
      • TNSALP
      see all

    Associated products

      Images

      • Example Standard Curve
        Example Standard Curve

        Alkaline phosphatase dose response was measured with Alkaline Phosphatase Assay Kit (Luminometric) (ab233466) in a solid white 96-well plate using a NovoStar microplate reader (BMG Labtech). As low as 0.001 mU/mL alkaline phosphatase can be detected with 20 minutes incubation (n=3).

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab233466? Please let us know so that we can cite the reference in this datasheet.

      ab233466 has not yet been referenced specifically in any publications.

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