Key features and details
- Assay type: Quantitative
- Detection method: Luminescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Product nameAlkaline Phosphatase Assay Kit (Luminometric)
See all Alkaline Phosphatase kits
Sample typeAdherent cells, Suspension cells
Alkaline Phosphatase Assay Kit (ab233466) uses D-luciferin phosphate as the luminogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions and cells.
D-luciferin phosphate is not recognized by luciferase until its phosphate group is removed to give luciferin. This kit provides all the essential components with an optimized assay protocol which is compatible with HTS liquid-handling instruments. The assay can be readily performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
The high sensitivity makes the kit ideal for the assays that require low detection limit.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Alkaline Phosphatase Standard 1 vial Assay Buffer 1 x 5ml Phosphatase Substrate 1 vial Reaction Buffer 1 x 5ml
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase, germ cell
- Alkaline phosphatase, testicular and thymus
ab233466 has not yet been referenced specifically in any publications.