Alkaline Phosphatase Anti-Biotin antibody (ab6652)
Key features and details
- Alkaline Phosphatase Goat polyclonal to Biotin
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-Biotin antibody
See all Biotin primary antibodies -
Description
Alkaline Phosphatase Goat polyclonal to Biotin -
Host species
Goat -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule conjugated to KLH
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General notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab6652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| WB | 1/1000 - 1/3000. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Protocols
References (1)
ab6652 has been referenced in 1 publication.
- Paul A et al. Streptavidin-coated magnetic beads for DNA strand separation implicate a multitude of problems during cell-SELEX. Oligonucleotides 19:243-54 (2009). WB . PubMed: 19732022
