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    alkaline-phosphatase-biotin-antibody-ab6652.pdf

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Alkaline Phosphatase Anti-Biotin antibody (ab6652)

  • Datasheet
  • SDS
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Key features and details

  • Alkaline Phosphatase Goat polyclonal to Biotin
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
  • Reacts with: Species independent
  • Conjugation: Alkaline Phosphatase
  • Isotype: IgG

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Overview

  • Product name

    Alkaline Phosphatase Anti-Biotin antibody
    See all Biotin primary antibodies
  • Description

    Alkaline Phosphatase Goat polyclonal to Biotin
  • Host species

    Goat
  • Conjugation

    Alkaline Phosphatase
  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH

  • General notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6652 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    WB
    1/1000 - 1/3000.
    Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    WB
    1/1000 - 1/3000.

    Target

    • Relevance

      Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • Alternative names

      • Vitamin B4 antibody

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab6652? Please let us know so that we can cite the reference in this datasheet.

    ab6652 has been referenced in 1 publication.

    • Paul A  et al. Streptavidin-coated magnetic beads for DNA strand separation implicate a multitude of problems during cell-SELEX. Oligonucleotides 19:243-54 (2009). WB . PubMed: 19732022

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