Alkaline Phosphatase chromogen (BCIP/TNBT) (ab7413)
Overview
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Product name
Alkaline Phosphatase chromogen (BCIP/TNBT)
See all Alkaline Phosphatase Chromogen reagents -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
General notes
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
Constituents: 0.02% BCIP, 0.04% TNBT -
Concentration information loading...
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Reagent notes
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab7413 has not yet been referenced specifically in any publications.