Product nameAlkaline Phosphatase chromogen (BCIP/TNBT)
See all Alkaline Phosphatase Chromogen reagents
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
Storage instructionsStore at +4°C.
Storage bufferpH: 9.20
Constituents: 0.02% BCIP, 0.04% TNBT
Concentration information loading...
Reagent notesBCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
Our Abpromise guarantee covers the use of ab7413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab7413 has not yet been referenced specifically in any publications.