Alkaline Phosphatase chromogen (BCIP/TNBT) (ab7413)


  • Product name
    Alkaline Phosphatase chromogen (BCIP/TNBT)
    See all Alkaline Phosphatase Chromogen reagents
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • General notes

    This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.

    BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".

    BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.



Our Abpromise guarantee covers the use of ab7413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


ab7413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

I am sorry to confirm that ab133406 is not suitable for detection of AP. This detection system has been designed for optimal detection of HRP, and will not be as sensitive for detection of AP.

I am sorr...

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Thank you for contacting us.

As requested, I have attached the Certificates of Compliance for the antibodies and reagents you mentioned - to this email.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Thank you for confirming the order number.
This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab64268 (new and different Batch:GR71729-1) as a free of charge replacement. I hope the ...

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Thank you for getting back to me and for re-testing the ALP (AP) activity with new and old chromogen solution. Your co-operation and understanding in this matter is very much appreciated!
I can certainly replace the faulty product (ab64268) as soo...

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Thank you very much for getting back to me promptly and for checking the ALP (AP) activity.
This is very important piece of detail and it may indeed imply that AP is not working properly. As I understand Streptavidin Alkaline Phosphatase (ab64268)...

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Thank you very much for confirming the order details.
Could you please comment on if the ALP (AP) enzyme conjugated to the antibody is inactive? Have you had any chance to check if the ALP is still functioning?
Would you prefer to get a new p...

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Thank you for your enquiry regarding ab7413 and for taking the time to provide some very useful details of the experiments. Your time and effort is much appreciated!
I am very sorry to hear that you are having problems with this chromogen.

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Thank you for contacting us.  BCIP/TNBT (ab7413) is similar to BCIP/NBT (ab7468) but is more sensitive and produces a more intense purple color.  Otherwise, the two products will be interchangeable.  I hope this helps, please let me know if you need an...

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Thank you very much for your enquiry. We do not currently sell substrates such as AP and HRP that have been tested in ELISPOT. This doesn’t mean that these products are not suitable, we just have not tested them in this application. The 4 produc...

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This substrate would detect any endogenous alkaline phosphatase if it were enzymatically active, but I am not sure how activity could remain since alkaline phosphatase is normally inactivated by freezing and chemical treatment during fixation.


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