Product nameAlkaline phosphatase Conjugation Kit
Alkaline Phosphatase Conjugation Kit ab102850 uses a simple and quick process to conjugate an antibody to Alkaline Phosphatase. It can also be used to conjugate other proteins or peptides.
To conjugate an antibody to Alkaline Phosphatase using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to HRP.
Amount and volume of antibody for conjugation to Alkaline Phosphatase
Kit size Recommended maximum
amount of antibody
30 µg 3 x 10 µg 3 x 10 µL 100 µg 100 µg 100 µL 300 µg 3 x 100 µg 3 x 100 µL 1 mg 1 mg 1 mL
1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Tested applicationsSuitable for: Conjugationmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 300 µg 1 mg 100 µg 30 µg AP-Mix 3 x 100µg 1 x 1mg 1 x 100µg 3 x 10µg Modifier reagent 1 vial 1 vial 1 vial 1 vial Quencher reagent 1 vial 1 vial 1 vial 1 vial
Our Abpromise guarantee covers the use of ab102850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent dilution.|
This product has been referenced in:
- Feng L et al. Human cytomegalovirus UL23 inhibits transcription of interferon-? stimulated genes and blocks antiviral interferon-? responses by interacting with human N-myc interactor protein. PLoS Pathog 14:e1006867 (2018). Read more (PubMed: 29377960) »
- Lo Passo C et al. Molecular characterization of two sub-family specific monoclonal antibodies to meningococcal Factor H binding protein. Heliyon 4:e00591 (2018). Read more (PubMed: 29644339) »