Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
![Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)](https://www.abcam.com/ps/products/81/ab81655/Images/ab81655-457459-alkaline-phosphatase-anti-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-western-blot.jpg "Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)")
Key features and details
- Alkaline Phosphatase Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Overview
-
Product name
Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Specificity
ab81655 recognizes the DYKDDDDK tag sequence (also known as the FLAG® tag sequence) at an N-terminal, Met-N-terminal, C-terminal, or internal site.
-
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
Sequence:DYKDDDDK
-
General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.3025% Tris, 0.87% Sodium chloride -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
M2 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
- SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000.
|
| Notes |
|---|
|
WB
1/5000. |
Target
-
Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
-
Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)All lanes : Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655) at 1/10000 dilution
Lane 1 : 60 kDa DYKDDDDK-tagged protein at 0.3 µg
Lane 2 : 60 kDa DYKDDDDK-tagged protein at 0.01 µg
Lane 3 : 60 kDa DYKDDDDK-tagged protein at 0.03 µg
Lane 4 : 60 kDa DYKDDDDK-tagged protein at 0.001 µg
Lane 5 : 60 kDa DYKDDDDK-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
Datasheets and documents
-
Datasheet download
References (0)
ab81655 has not yet been referenced specifically in any publications.
