Alkaline Phosphatase Anti-Digoxigenin antibody [21H8] (ab119345)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-Fr, Southern Blot, ICC/IF
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Overview
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Product name
Alkaline Phosphatase Anti-Digoxigenin antibody [21H8]
See all Digoxigenin primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [21H8] to Digoxigenin -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Specificity
ab119345 reacts with free and bound digoxigenin. It is useful for detection of digoxigenin labelled compounds. -
Tested applications
Suitable for: WB, ELISA, IHC-Fr, Southern Blot, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Digoxigenin
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 50% Glycerol, 0.121% Tris, 0.81% Sodium chloride, 0.01% Magnesium chloride, 0.014% Zinc chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
21H8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab119345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. | |
| ELISA | 1/500 - 1/5000. | |
| IHC-Fr | 1/200 - 1/2000. | |
| Southern Blot | 1/500 - 1/5000. | |
| ICC/IF | 1/200 - 1/2000. |
Target
-
Relevance
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
Protocols
References (6)
ab119345 has been referenced in 6 publications.
- Zhang H et al. Serum exosomes mediate delivery of arginase 1 as a novel mechanism for endothelial dysfunction in diabetes. Proc Natl Acad Sci U S A 115:E6927-E6936 (2018). PubMed: 29967177
- Liu Y et al. Cystic fibrosis transmembrane conductance regulator mediates tenogenic differentiation of tendon-derived stem cells and tendon repair: accelerating tendon injury healing by intervening in its downstream signaling. FASEB J 31:3800-3815 (2017). PubMed: 28495756
- Ohana G et al. A3 adenosine receptor agonist, CF102, protects against hepatic ischemia/reperfusion injury following partial hepatectomy. Mol Med Rep 14:4335-4341 (2016). PubMed: 27666664
- Liang DY et al. Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis. Int J Mol Med 38:131-8 (2016). IHC ; Human . PubMed: 27246196
- Li DQ et al. Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma. Oncol Lett 11:3003-3008 (2016). PubMed: 27123053
- Parry L et al. Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models. J Vis Exp N/A:N/A (2015). PubMed: 26649885
