Alkaline Phosphatase Anti-Fluorescein antibody (ab6657)

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Key features and details

  • Alkaline Phosphatase Goat polyclonal to Fluorescein
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
  • Reacts with: Species independent
  • Conjugation: Alkaline Phosphatase
  • Isotype: IgG

Overview

  • Product name

    Alkaline Phosphatase Anti-Fluorescein antibody
    See all Fluorescein primary antibodies

  • Description

    Alkaline Phosphatase Goat polyclonal to Fluorescein

  • Host species

    Goat

  • Conjugation

    Alkaline Phosphatase

  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Fluorescein conjugated to Goat IgG

  • General notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab6657 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Dot blot 1/1000 - 1/5000.
    ELISA 1/1000 - 1/5000.
    IHC-P 1/1000 - 1/5000.
    IHC-Fr 1/1000 - 1/5000.
    WB 1/1000 - 1/5000.

    Target

    • Relevance

      Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.

    • Alternative names

      • FI antibody
      • FITC antibody
      • fluorescein isothyocianate antibody
      • fluorescyl antibody

    References (0)

    Publishing research using ab6657? Please let us know so that we can cite the reference in this datasheet.

    ab6657 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Can you please provide me with dilution guidelines for ab6657 polyclonal to fluorescein (alkaline phosphatase) for use in ELISA using o-nitrophenyl phosphate as a substrate. Thanks Phil Duncanson

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    Answer

    Based on the Lot #6189 of ab6657, a working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product (assayed against 1.0 ug of Fluorescein conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature)

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