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Tags & Cell Markers Fusion / Marker Proteins GFP
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Alkaline Phosphatase Anti-GFP antibody (ab6661)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (1)

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Abpromise

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Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
  • Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)

Key features and details

  • Alkaline Phosphatase Goat polyclonal to GFP
  • Suitable for: WB, ELISA, Dot blot
  • Reacts with: Species independent
  • Conjugation: Alkaline Phosphatase
  • Isotype: IgG

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Overview

  • Product name

    Alkaline Phosphatase Anti-GFP antibody
    See all GFP primary antibodies
  • Description

    Alkaline Phosphatase Goat polyclonal to GFP
  • Host species

    Goat
  • Conjugation

    Alkaline Phosphatase
  • Specificity

    Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.
  • Tested applications

    Suitable for: WB, ELISA, Dot blotmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Fusion protein corresponding to GFP aa 1-246.
    Database link: P42212

  • General notes

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, anti-Alkaline Phosphatase and purified and partially purified GFP (Aequorea victoria) serum.
  • Primary antibody notes

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP
    • Signal Transduction
    • Antibodies
    • gfp

Associated products

  • Recombinant Protein

    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • GFP ELISA Kit (ab171581)

Applications

Our Abpromise guarantee covers the use of ab6661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500.
ELISA 1/2000 - 1/8000.

This product has been assayed against 1.0 µg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.

Dot blot 1/1000.

Target

  • Relevance

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names

    • GFP antibody
    • Green fluorescent protein antibody

Images

  • Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
    Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
    Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution + GFP at 0.05 µg with Blocking buffer
  • Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
    Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)

    Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.

    Lane 1: 100 ng GFP protein
    Lane 2: 33.33 ng GFP protein
    Lane 3: 11.11 ng GFP protein
    Lane 4: 3.70 ng GFP protein
    Lane 5: 1.23 ng GFP protein

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab6661? Please let us know so that we can cite the reference in this datasheet.

    ab6661 has been referenced in 1 publication.

    • Oravcová M  et al. Fission yeast CSL proteins function as transcription factors. PLoS One 8:e59435 (2013). WB . PubMed: 23555033

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Does this antibody detect GFP on western blots of tissue homogenates? In flies?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 27 2005

    Answer

    Thank you for your enquiry. This antibody is suitable for use in Western blotting. It shows a 42 kDa band when reacted with GFP on a Western blot, and the antibody recognizes wild type, recombinant and enhanced forms of GFP. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Aug 31 2005

    Question

    I have GFP-tagged a cytokine and I would like to be able to detect cytokine binding to the receptor. Your report that this antibody can detect 1 ug GFP in an ELISA not very impressive. Any idea what the limit of detection might be?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2001

    Answer

    The ELISA data is using 1ug to coat the wells and is used as a control not as a minimum level of detection. Unfortunately we have no data on the detection limit in ELISA.

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2001

    Question

    Has anyone ever tried using this antibody for immunihistochemistry? It doesn't say that it's appropriate for IHC, but I don't know if that's because noone has ever tried, or because it doesn't work.

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2001

    Answer

    To our knowledge this product has yet to be tested in immunihistochemistry. We are very keen for information regarding this application.

    Read More

    Abcam Scientific Support

    Answered on May 18 2001

    Question

    Do you have a protocol for a sandwich ELISA using this GFP Ab (6661)?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2001

    Answer

    You can use any standard sandwich ELISA protocol. Use ab6673 (goat anti-GFP) as capturing antibody at 1 microg/well and ab6661 at a dilution of 1:1,000 to 1:5,000.

    Read More

    Abcam Scientific Support

    Answered on Mar 22 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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