Alkaline Phosphatase Anti-GST antibody (ab106225)
Key features and details
- Alkaline Phosphatase Rabbit polyclonal to GST
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-GST antibody
See all GST primary antibodies -
Description
Alkaline Phosphatase Rabbit polyclonal to GST -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Specificity
Assay by immunoelectrophoresis resulted in a single precipition arc against anti-Rabbit Serum, as well as purified and partially purified GST [Schistosoma japonicum]. Cross reactivity against GST from other sources may occur but has not been specifically determined. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length GST isolated from Schistosoma japonicum.
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Positive control
- Recombinant GST protein.
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General notes
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.01% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA
BSA is Immunoglobulin and Protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106225 was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 26 kDa. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Alkaline Phosphatase Anti-GST antibody (ab106225)Alkaline Phosphatase Anti-GST antibody (ab106225) at 1/1000 dilution + Recombinant GST protein at 0.25 µg
Secondary
HRP conjugated Goat Anti-Rabbit at 1/40000 dilution
Predicted band size: 26 kDa
Datasheets and documents
References (0)
ab106225 has not yet been referenced specifically in any publications.
