Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)
![Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)](https://www.abcam.com/ps/products/81/ab81656/Images/ab81656-457460-alkaline-phosphatase-anti-ha-tag-antibody-16b12-western-blot.jpg "Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)")
Key features and details
- Alkaline Phosphatase Mouse monoclonal [16B12] to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Overview
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Product name
Alkaline Phosphatase Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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Epitope
The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000.
|
| Notes |
|---|
|
WB
1/5000. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)All lanes : Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656) at 1/10000 dilution
Lane 1 : 60 kDa HA-tagged protein at 0.3 µg
Lane 2 : 60 kDa HA-tagged protein at 0.01 µg
Lane 3 : 60 kDa HA-tagged protein at 0.03 µg
Lane 4 : 60 kDa HA-tagged protein at 0.001 µg
Lane 5 : 60 kDa HA-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
Datasheets and documents
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Datasheet download
References (0)
ab81656 has not yet been referenced specifically in any publications.
