Key features and details
- Alkaline Phosphatase Mouse monoclonal [16B12] to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Product nameAlkaline Phosphatase Anti-HA tag antibody [16B12]
See all HA tag primary antibodies
DescriptionAlkaline Phosphatase Mouse monoclonal [16B12] to HA tag
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
EpitopeThe HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
All lanes : Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656) at 1/10000 dilution
Lane 1 : 60 kDa HA-tagged protein at 0.3 µg
Lane 2 : 60 kDa HA-tagged protein at 0.01 µg
Lane 3 : 60 kDa HA-tagged protein at 0.03 µg
Lane 4 : 60 kDa HA-tagged protein at 0.001 µg
Lane 5 : 60 kDa HA-tagged protein at 0.003 µg
Imaged using stabilized Alkaline Phosphatase substrate.
ab81656 has not yet been referenced specifically in any publications.