Alkaline Phosphatase Anti-S tag antibody (ab133157)
Key features and details
- Alkaline Phosphatase Rabbit polyclonal to S tag
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-S tag antibody
See all S tag primary antibodies -
Description
Alkaline Phosphatase Rabbit polyclonal to S tag -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Prior to use, reconstitute to 1 mL with ddH2O, vortex gently and allow material to reconstitute for 20 minutes. Avoid exposure to heat. After product has been reconstituted, store at 2-8C in the dark and use within 6 months. For extended storage, add an equal volume of glycerol (ACS grade) for a final concentration of 50%, and store at -20 degrees Celsius. Prepare a working dilution daily. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.3% Tris, 0.88% Sodium chloride, 0.047% Magnesium chloride, 0.0016% Zinc chloride -
Concentration information loading...
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Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/50000. Starting dilution: 1/2000
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ELISA |
1/5000 - 1/50000.
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Notes |
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WB
1/5000 - 1/50000. Starting dilution: 1/2000 |
ELISA
1/5000 - 1/50000. |
Target
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Relevance
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. -
Alternative names
- KETAAAKFERQHMDS tag antibody antibody
- S peptide antibody
- S peptide epitope tag antibody
Images
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All lanes : Alkaline Phosphatase Anti-S tag antibody (ab133157) at 1/10000 dilution
Lane 1 : 60 kDa S-tagged protein at 0.3 µg
Lane 2 : 60 kDa S-tagged protein at 0.01 µg
Lane 3 : 60 kDa S-tagged protein at 0.03 µg
Lane 4 : 60 kDa S-tagged protein at 0.001 µg
Lane 5 : 60 kDa S-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133157 has not yet been referenced specifically in any publications.