Product nameAlkaline Phosphatase Staining Kit (Red)
See all Alkaline Phosphatase kits
Alkaline Phosphatase Staining Kit (Red) (ab242286) uses a convenient method to identify and monitor stem cells by staining for alkaline phosphatase activity.
Pluripotent stem cells typically have higher levels of alkaline phosphatase activity than differentiated cell types (although osteoblasts retain this activity). Alkaline phosphatase staining / activity is often used as a biomarker of stem cells.
In this alkaline phosphatase staining kit, alkaline phosphatase acts on a substrate to form a red colored chemical, producing a red color in stem cells that is imaged with light microscopy.
The kit can be used with any tissue section or cell type that have alkaline phosphatase activity. It is not compatible with paraffin-embedded sections.
The alkaline phosphatase staining protocol is simple:
- prepare samples (the kit protocol is written for use with ES cells; the kit can be used with any cell or tissues with alkaline phosphatase activity)
- add fixing solution to cells or tissues, incubate for 2 mins, and wash with PBST
- add staining solution and incubate for 15-30 mins
- wash with PBS and image
Alkaline Phosphatase Staining Kit (Purple) ab242287 is an alternative to this kit, using an equivalent method, with a different colored dye. Generally, this kit gives cleaner staining.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests AP Staining Solution A 1 x 20ml AP Staining Solution B 1 x 20ml Fixing Solution 1 x 50ml
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Alkaline phosphatase, germ cell
- Alkaline phosphatase, testicular and thymus
Murine embryonic stem cells (ES-D3) are maintained in an undifferentiated stage on gelatin-coated dishes in the presence of LIF, as indicated by the high AP activity. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, AP staining of undifferentiated cells was performed as described in the Assay Protocol.
ab242286 has not yet been referenced specifically in any publications.