Alkaline Phosphatase Anti-T7 tag® antibody (ab133151)
Key features and details
- Alkaline Phosphatase Rabbit polyclonal to T7 tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
Alkaline Phosphatase Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide:
MASMTGGQQMG
, corresponding to amino acids 1-11 of T7 tag®
Properties
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Form
Lyophilized:Reconstitute to 1 mL with ddH2O, vortex gently and allow material to reconstitute for 20 minutes, avoid exposure to heat. After product has been reconstituted, product should be stored at 2-8 C in the dark and be used within 6 months. For extended storage, add an equal volume of glycerol (ACS grade) for a final concentration of 50%, and store at -20 degrees Celsius. Prepare a working dilution daily. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.3% Tris, 0.88% Sodium chloride, 0.05% Magnesium chloride, 0.0016% Zinc chloride -
Concentration information loading... -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/50000.
|
|
| ELISA |
1/5000 - 1/50000.
|
| Notes |
|---|
|
WB
1/5000 - 1/50000. |
|
ELISA
1/5000 - 1/50000. |
Target
-
Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Images
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Western blot - Alkaline Phosphatase Anti-T7 tag® antibody (ab133151)All lanes : Alkaline Phosphatase Anti-T7 tag® antibody (ab133151) at 1/10000 dilution
Lane 1 : 60 kDa T7-tagged protein at 0.3 µg
Lane 2 : 60 kDa T7-tagged protein at 0.01 µg
Lane 3 : 60 kDa T7-tagged protein at 0.03 µg
Lane 4 : 60 kDa T7-tagged protein at 0.001 µg
Lane 5 : 60 kDa T7-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
References (0)
ab133151 has not yet been referenced specifically in any publications.
