Overview

  • Product name

    Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description

    Goat polyclonal to Alkaline Phosphatase, Tissue Non-Specific
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize isoform 1 (NP_000469.3) only.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 42-53 (N terminal) (Cysteine residue). NP_000469.3.
    Sequence:

    C-ELQKLNTNVAKN


    Database link: P05186

  • Positive control

    • WB: Human kidney, lung and adrenal gland lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223497 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57.3 kDa).

Target

  • Function

    This isozyme may play a role in skeletal mineralization.
  • Involvement in disease

    Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Images

  • All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab223497) at 0.1 µg/ml

    Lane 1 : Human kidney lysate in RIPA buffer
    Lane 2 : Human lung lysate in RIPA buffer
    Lane 3 : Human adrenal gland lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57.3 kDa



    Primary incubation was 1 hour.

References

ab223497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223497.
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