Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies -
Description
Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100 (N terminal).
Database link: P05186 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA, 0.45% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
This isozyme may play a role in skeletal mineralization. -
Involvement in disease
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
Sequence similarities
Belongs to the alkaline phosphatase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 249 Human
- Entrez Gene: 25586 Rat
- Omim: 171760 Human
- SwissProt: P05186 Human
- SwissProt: P08289 Rat
- Unigene: 75431 Human
- Unigene: 82764 Rat
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Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Images
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All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 0.5 µg/ml
Lane 1 : Human Placenta Tissue Lysate at 50 µg
Lanes 2-3 : JURKAT Whole Cell Lysate at 40 µg
Predicted band size: 57 kDa -
This image shows ab65834 at 1-2µg/ml staining cytoplasmic Alkaline Phosphatase in rat small intestine tissue.
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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 2 µg/ml + HeLa cell lysate
Predicted band size: 57 kDa
Observed band size: 57 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab65834 has been referenced in 33 publications.
- Zhang Y et al. Applying exercise-mimetic engineered skeletal muscle model to interrogate the adaptive response of irisin to mechanical force. iScience 25:104135 (2022). PubMed: 35434556
- Sun X et al. Paeoniflorin Ameliorates Hyperprolactinemia-Induced Inhibition of Osteoblastogenesis by Suppressing the NF-κB Signaling Pathway. Int J Endocrinol 2022:4572033 (2022). PubMed: 35465073
- Yan C et al. Extracellular vesicles from the inflammatory microenvironment regulate the osteogenic and odontogenic differentiation of periodontal ligament stem cells by miR-758-5p/LMBR1/BMP2/4 axis. J Transl Med 20:208 (2022). PubMed: 35562763
- Wu M et al. JAM-A facilitates hair follicle regeneration in alopecia areata through functioning as ceRNA to protect VCAN expression in dermal papilla cells. Precis Clin Med 5:pbac020 (2022). PubMed: 36132055
- Wang Q et al. miR-153-3p inhibits osteogenic differentiation of BMSCs by down-regulating the expression of RUNX2 in a high glucose environment. Am J Transl Res 14:7027-7039 (2022). PubMed: 36398274