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Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Alkaline Phosphatase, Tissue Non-Specific.
- This antibody gave a positive signal in Human kidney tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
This isozyme may play a role in skeletal mineralization.
Involvement in disease
Hypophosphatasia childhood type
Hypophosphatasia infantile type
Belongs to the alkaline phosphatase family.
Information by UniProt
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789)
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203
) at 10 µgSecondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
57 kDaObserved band size:
57 kDaAdditional bands at:
48 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"