Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab97384)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies -
Description
Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela, HepG2, MOLT4 and Raji cell lines; OVCAR3 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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IHC-P | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
This isozyme may play a role in skeletal mineralization. -
Involvement in disease
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
Sequence similarities
Belongs to the alkaline phosphatase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 727689 Cat
- Entrez Gene: 280994 Cow
- Entrez Gene: 249 Human
- Entrez Gene: 11647 Mouse
- Entrez Gene: 25586 Rat
- Omim: 171760 Human
- SwissProt: Q29486 Cat
- SwissProt: P09487 Cow
see all -
Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Images
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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab97384) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded OVCAR3 xenograft, using ab97384 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97384 has been referenced in 2 publications.
- Lionikaite V et al. Vitamin A decreases the anabolic bone response to mechanical loading by suppressing bone formation. FASEB J 33:5237-5247 (2019). PubMed: 30668919
- Ren T et al. CD49b inhibits osteogenic differentiation and plays an important role in osteosarcoma progression. Oncotarget 8:87848-87859 (2017). PubMed: 29152125