Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (HRP)
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
DescriptionRabbit monoclonal [EPR4477] to Alkaline Phosphatase, Tissue Non-Specific (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100. The exact sequence is proprietary.
Database link: P05186
- WB: SAOS2, HepG2 and HeLa whole cell lysate (ab150035). IHC-P: FFPE human liver (normal) tissue sections.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 57 kDa).|
FunctionThis isozyme may play a role in skeletal mineralization.
Involvement in diseaseHypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
IHC image of Alkaline Phosphatase, Tissue Non-specific staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab200895 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (HRP) (ab200895) at 1/5000 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200895 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200895 has not yet been referenced specifically in any publications.