Recombinant
RabMAb

Recombinant Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] - Low endotoxin, Azide free (ab218574)

Overview

  • Product name

    Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] - Low endotoxin, Azide free
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description

    Rabbit monoclonal [EPR4477] to Alkaline Phosphatase, Tissue Non-Specific - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICC, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100. The exact sequence is proprietary.
    Database link: P05186

  • Positive control

    • WB: SAOS2, HepG2, HeLa, and JAR cell lysates IHC-P: Human kidney and liver tissues ICC/IF: HeLa cells
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Function

    This isozyme may play a role in skeletal mineralization.
  • Involvement in disease

    Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Images

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human kidney tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108337).

  • Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108337).

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human liver tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108337).

  • ab108337, at a 1/100 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108337).

References

This product has been referenced in:

  • Du M  et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017). WB ; Rat . Read more (PubMed: 27770935) »
  • Huang X  et al. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro. Mol Med Rep 13:613-22 (2016). WB ; Mouse . Read more (PubMed: 26648136) »
See all 4 Publications for this product

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