Western blot abreview for Anti-ALKBH1 antibody [EPR19215]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293T total cell lysate)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
40 µg
Treatment
control siRNA transfection- cells transfected with a random siRNA sequence for 48 hrs
Specification
HEK-293T total cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Chemiluminescence
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 43 kDa Non-specific: 68 kDa
Positive control
The three lanes shown on the picture (Lanes 1-3) are three biological replicates of HEK-293T cells transfected with a random siRNA sequence for 48 hrs. These three samples serve as a negative control for an AlkBH1 KD test while the KD samples are not shown.

Abcam user community

Verified customer

Submitted Jun 02 2018

Sign up