Overview

  • Product name
    Anti-ALKBH1 antibody [EPR6175(2)]
    See all ALKBH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6175(2)] to ALKBH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ALKBH1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • A431, Jurkat, A549, K562, Raw 264.7, and NIH3T3 cell lysates, Human breast carcinoma tissue
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABH antibody
      • ABH1 antibody
      • alkB antibody
      • alkB homolog 1 histone H2A dioxygenase antibody
      • AlkB, alkylation repair homolog 1 (E. coli) antibody
      • ALKB1_HUMAN antibody
      • ALKBH antibody
      • ALKBH1 antibody
      • Alkylated DNA repair protein alkB homolog 1 antibody
      • Alkylation repair homolog 1 antibody
      • Alkylation repair, alkB homolog antibody
      • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
      • DNA lyase ABH1 antibody
      • DNA oxidative demethylase ALKBH1 antibody
      • hABH antibody
      see all

    Images

    • All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    • ab128895, at a dilution of 1/50, staining ALKBH1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells total cell lysate)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    40 µg
    Treatment
    control siRNA transfection- cells transfected with a random siRNA sequence for 48 hrs
    Specification
    HEK293T cells total cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Jun 02 2018

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