Product nameAnti-ALKBH1 antibody [EPR6175(2)]
See all ALKBH1 primary antibodies
DescriptionRabbit monoclonal [EPR6175(2)] to ALKBH1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ALKBH1 aa 1-100 (N terminal). The exact sequence is proprietary.
- A431, Jurkat, A549, K562, Raw 264.7, and NIH3T3 cell lysates, Human breast carcinoma tissue
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionDioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
Sequence similaritiesBelongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Cellular localizationMitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
- Information by UniProt
- ABH antibody
- ABH1 antibody
- alkB antibody
All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
ab128895, at a dilution of 1/50, staining ALKBH1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
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