Overview

  • Product name
    Anti-ALKBH1 antibody [EPR6175(2)] - BSA and Azide free
    See all ALKBH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6175(2)] to ALKBH1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ALKBH1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    ab248205 is a PBS-only buffer format of ab128895. Please refer to ab128895 for recommended dilutions, protocols, and image data.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab248205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABH antibody
      • ABH1 antibody
      • alkB antibody
      • alkB homolog 1 histone H2A dioxygenase antibody
      • AlkB, alkylation repair homolog 1 (E. coli) antibody
      • ALKB1_HUMAN antibody
      • ALKBH antibody
      • ALKBH1 antibody
      • Alkylated DNA repair protein alkB homolog 1 antibody
      • Alkylation repair homolog 1 antibody
      • Alkylation repair, alkB homolog antibody
      • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
      • DNA lyase ABH1 antibody
      • DNA oxidative demethylase ALKBH1 antibody
      • hABH antibody
      see all

    References

    ab248205 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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