Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6176] to ALKBH1 - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-ALKBH1 antibody [EPR6176] - BSA and Azide free
See all ALKBH1 primary antibodies
DescriptionRabbit monoclonal [EPR6176] to ALKBH1 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human ALKBH1 aa 350 to the C-terminus. The exact sequence is proprietary.
ab248120 is the carrier-free version of ab126596 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 2.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab248120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
FunctionDioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
Sequence similaritiesBelongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Cellular localizationMitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
- Information by UniProt
- ABH antibody
- ABH1 antibody
- alkB antibody
Lanes 1-2 : Anti-ALKBH1 antibody [EPR6176] - BSA and Azide free (ab248120) at 1/1000 dilution
Lanes 3-4 : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126596).
ab126596 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab126596 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248120 has not yet been referenced specifically in any publications.