Overview

  • Product name
    Anti-ALKBH1 antibody [EPR6176] - BSA and Azide free
    See all ALKBH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6176] to ALKBH1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ALKBH1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • General notes

    ab248120 is a PBS-only buffer format of ab126596. Please refer to ab126596 for recommended dilutions, protocols, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab248120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABH antibody
      • ABH1 antibody
      • alkB antibody
      • alkB homolog 1 histone H2A dioxygenase antibody
      • AlkB, alkylation repair homolog 1 (E. coli) antibody
      • ALKB1_HUMAN antibody
      • ALKBH antibody
      • ALKBH1 antibody
      • Alkylated DNA repair protein alkB homolog 1 antibody
      • Alkylation repair homolog 1 antibody
      • Alkylation repair, alkB homolog antibody
      • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
      • DNA lyase ABH1 antibody
      • DNA oxidative demethylase ALKBH1 antibody
      • hABH antibody
      see all

    References

    ab248120 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab248120.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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