Overview

  • Product name
    Anti-ALKBH2 antibody [EPR6177(2)]
    See all ALKBH2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6177(2)] to ALKBH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ALKBH2.
    Database link: Q6NS38

  • Positive control
    • Human fetal brain, Human testis, Jurkat and HeLa lysates; Jurkat cells.
  • General notes

     

     

    Previously labelled as ABH2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
    • Tissue specificity
      Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Nucleus. Detected in replication foci during S-phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2OG Fe(II) oxy DC1 antibody
      • 9530023G02 antibody
      • ABH 2 antibody
      • ABH2 antibody
      • alkB alkylation repair homolog 2 antibody
      • ALKB2_HUMAN antibody
      • ALKBH2 alkB antibody
      • ALKBH2 antibody
      • Alkylated DNA repair protein alkB homolog antibody
      • Alkylated DNA repair protein alkB homolog 2 antibody
      • Alkylation repair homolog 2 antibody
      • Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody
      • AU016977 antibody
      • DNA oxidative demethylase ALKBH2 antibody
      • EC 1.14.11. antibody
      • FLJ99103 antibody
      • hABH2 antibody
      • mABH2 antibody
      • MGC90512 antibody
      • Oxy DC1 antibody
      see all

    Images

    • All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution

      Lanes 1 & 3 : Human fetal brain tissue lysate
      Lane 2 : Human testis tissue lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling ALKBH2 with ab154859 at 1/100 (red). Rabbit IgG control (green).

    References

    ab154859 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154859.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up