Anti-ALKBH3 antibody (ab220403)

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Overview

  • Product name

    Anti-ALKBH3 antibody
    See all ALKBH3 primary antibodies

  • Description

    Rabbit polyclonal to ALKBH3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human ALKBH3 aa 151-263.
    Sequence:

    NPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGR CPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGA TQADWQHRVPKEY


    Database link: Q96Q83

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF - A431 cells

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab220403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.

  • Tissue specificity

    Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.

  • Sequence similarities

    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1700108H04Rik antibody
    • 1810020C19Rik antibody
    • ABH3 antibody
    • AlkB homolog 3 antibody
    • alkB, alkylation repair homolog 3 (E. coli) antibody
    • ALKB3_HUMAN antibody
    • ALKBH3 antibody
    • Alkylated DNA repair protein alkB homolog 3 antibody
    • alkylation repair homolog 3 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 antibody
    • DEPC 1 antibody
    • DEPC-1 antibody
    • DEPC1 antibody
    • EC 1.14.11. antibody
    • FLJ43614 antibody
    • mABH3 antibody
    • MGC118790 antibody
    • MGC118792 antibody
    • MGC118793 antibody
    • MGC134125 antibody
    • PCA1 antibody
    • Prostate cancer antigen 1 antibody
    • Prostate cancer antigen-1 antibody
    • RP23-375N21.4 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ALKBH3 antibody (ab220403)
    Immunocytochemistry/ Immunofluorescence - Anti-ALKBH3 antibody (ab220403)

    Immunofluorescent analysis of PFA fixed, Triton X-100 permeabilized A431 cells labeling ALKBH3 with ab220403 at 4 µg/ml (green).

References

ab220403 has not yet been referenced specifically in any publications.

Publishing research using ab220403? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-ALKBH3 antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 03 2018

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