Anti-ALKBH4 antibody (ab234802)
Key features and details
- Rabbit polyclonal to ALKBH4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ALKBH4 antibody
See all ALKBH4 primary antibodies -
Description
Rabbit polyclonal to ALKBH4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ALKBH4 aa 1-150.
Database link: Q9NXW9 -
Positive control
- WB: Mouse spleen, lung and kidney lysates. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/5000. Predicted molecular weight: 34 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/2000 - 1/5000. Predicted molecular weight: 34 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation. -
Tissue specificity
Widely expressed, with highest expression in pancreas, ovary and spleen. -
Sequence similarities
Belongs to the alkB family. -
Cellular localization
Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody. - Information by UniProt
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Database links
- Entrez Gene: 54784 Human
- Entrez Gene: 72041 Mouse
- Omim: 613302 Human
- SwissProt: Q9NXW9 Human
- SwissProt: Q9D8F1 Mouse
- Unigene: 658598 Human
- Unigene: 486605 Mouse
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Alternative names
- 2010004B12Rik antibody
- ABH4 antibody
- Abj4 antibody
see all
Images
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All lanes : Anti-ALKBH4 antibody (ab234802) at 1/2000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa -
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ALKBH4 (green) using ab234802 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234802 has not yet been referenced specifically in any publications.