Recombinant Anti-ALKBH4 antibody [EPR19216] (ab195379)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19216] to ALKBH4
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ALKBH4 antibody [EPR19216]
See all ALKBH4 primary antibodies -
Description
Rabbit monoclonal [EPR19216] to ALKBH4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, heart and kidney tissue lysate; human pancreas tissue lysate; HEK-293, MCF7, MOLT-4 and WI-38 whole cell lysate. IP: HEK-293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19216 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IP
1/30. |
Target
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Function
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation. -
Tissue specificity
Widely expressed, with highest expression in pancreas, ovary and spleen. -
Sequence similarities
Belongs to the alkB family. -
Cellular localization
Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody. - Information by UniProt
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Database links
- Entrez Gene: 54784 Human
- Omim: 613302 Human
- SwissProt: Q9NXW9 Human
- Unigene: 658598 Human
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Alternative names
- 2010004B12Rik antibody
- ABH4 antibody
- Abj4 antibody
see all
Images
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All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1 minuteBlocking/Dilution: 5% NFDM/TBST.
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All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 4 : Human pancreas tissue lysate
Lane 5 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1 minuteBlocking/Dilution: 5% NFDM/TBST.
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ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input).
Lane 2: ab195379 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab195379 has been referenced in 2 publications.
- Jingushi K et al. ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer. Sci Rep 11:8677 (2021). WB ; Human . PubMed: 33883577
- Zhou C et al. N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1. EBioMedicine 59:102955 (2020). PubMed: 32853985