Key features and details
- Rabbit polyclonal to ALKBH5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ALKBH5 antibody
See all ALKBH5 primary antibodies
DescriptionRabbit polyclonal to ALKBH5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- IHC-P: Human testis, kidney, liver and fallopian tube tissue. WB: U-87 MG cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.|
RelevanceDioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export and is required for spermatogenesis.
Cellular localizationNucleus speckle
- ABH5 antibody
- AlkB, alkylation repair homolog 5 (E. coli) antibody
- AlkB, alkylation repair homolog 5 antibody
Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for ALKBH5 with ab244296 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for ALKBH5 with ab244296 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for ALKBH5 with ab244296 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for ALKBH5 with ab244296 at a 1/500 dilution in immunohistochemical analysis.
Anti-ALKBH5 antibody (ab244296) at 0.4 µg/ml + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 44 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244296 has not yet been referenced specifically in any publications.