Overview

  • Product name
  • Description
    Rabbit polyclonal to ALKBH6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ALKBH6 aa 123-153 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q3KRA9

  • Positive control
    • Human MDA-MB435 cell line and mouse cerebellum tissue lysates; Human testis tissue; MDA-MB435 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170186 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 26 kDa.Can be blocked with ALKBH6 peptide (ab238351).
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificity
    Widely expressed, with highest expression in testis and pancreas.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH6 antibody
    • AlkB alkylation repair homolog 6 (E. coli) antibody
    • ALKB6_HUMAN antibody
    • alkbh6 antibody
    • Alkylated DNA repair protein alkB homolog 6 antibody
    • Alkylation repair homolog 6 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 antibody
    • Probable alpha ketoglutarate dependent dioxygenase ABH6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling ALKBH6 with ab170186 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-ALKBH6 antibody (ab170186) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg

    Predicted band size: 26 kDa

  • Anti-ALKBH6 antibody (ab170186) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg

    Predicted band size: 26 kDa

  • Flow cytometric analysis of MDA-MB435 cells, labeling ALKBH6 with ab170186 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170186 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170186.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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